Marine Theret
Name: Marine Theret
Position: Postdoctoral Research Fellow
Nationality: French
Research summary:
Beside its function as a biological barrier for infection, the immune system participates in multiple cellular process involved in tissue homeostasis during development, post-natal growth, regeneration/repair, and ageing. To do this, immune cells strongly interact with tissue-resident cells, such as Mesenchymal Progenitor (MPs). This is a two-way relationship, as MPs are also required to maintain organ function and tissue integrity, by strongly influencing immune cells inflammatory function. For example, a stable type 2 inflammatory (Th2) environment is an important, yet underestimated, parameter required for successful tissue maintenance. Indeed, white adipose tissue (WAT) resident MPs are responsible for the maintenance of a Th2 environment within the WAT at homeostasis. While the immune functions of MPs are actively studied, the signalling pathways involved in their regulation, which are likely to provide potential therapeutic targets, are so far poorly understood. MPs are the main cellular component of the tissue interstitial. Their role is to maintain the tissue homeostasis at resting state, but they are also crucial to keep the tissue integrity after a damage. While their function toward the ECM is well known, these cells have been more recently described as a key player of the inflammation.
During my PDF training, I have studied the implication of various signalling pathways involved on the inflammatory fate of the MPs.
Personal Summary:
Before moving to Vancouver, I would have described myself as a city person, enjoying museums, movies, and the night life. But the great outdoors of BC converted me to the west-coast life style (meaning that I cannot spend 2 days in a row without going to the gym, running, swimming, biking, hiking, camping, or driving across the province for a weekend). However (or luckily) my dry French humour is still very strong, and I still spend 60% of my time complaining.
Publications:
- Malakoutian M, Theret M, Yamamoto S, Dehghan-Hamani I, Lee M, Street J, Rossi F, Brown S, Oxland T. Larger Muscle Fibers and Fiber Bundles Manifest Smaller Elastic Modulus in Paraspinal Muscles of Rats and Humans. Sci Rep. 2021; 11(1):18565.
- Theret M*, Low M*, Rempel L, Li Fang Fang, Tung LW, Conreras O, Chang CK, Soliman H, Rossi FMV. In vitro assessment of anti-fibrotic drug activity does not predict in vivo efficacy in murine models of Duchenne muscular dystrophy. Life Sci. 2021; 279:119482. *authors contributed equally
- Yamamoto S, Malakoutian M, Theret M, Street J, Rossi FMV, Brown SHM, Oxland TR. The effect of posterior lumbar spinal surgery on passive stiffness of rat paraspinal muscles 13 weeks post-surgery. Spine (Phila Pa 1976). 2021; 46(21):E1125-E1135.
- White Z, Hakim CH, Theret M, Yang N, Rossi FMV, Cox D, Francis GA, Straub V, Selby K, Panagiotopoulos C, Duan D, Bernatchez P. High prevalence of plasma lipid abnormalities in human and canine Duchenne and Becker muscular dystrophies depicts a new type of primary genetic dyslipidemia. J Clin Lipidol. 2020; 14(4):459-469.e0.
- Contreras O, Soliman H, Theret M, Rossi FMV, Brandan E. TGF-β-driven downregulation of the transcription factor TCF7L2 affects Wnt/β-catenin signaling in PDGFRα+ fibroblasts. J Cell Sci. 2020; 133(12):jcs242297.
- McArthur S, Juban G, Gobbetti T, Desgeorges T, Theret M, Gondin J, Toller-Kawahisa JE, Reutelingsperger CP, Chazaud B, Perretti M, Mounier R. Annexin A1 drives macrophage skewing to accelerate muscle regeneration through AMPK activation J Clin Invest. 2020; 130(3):1156-1167.
- Contreras O, Cruz-Soca M, Theret M, Soliman H, Tung LW, Groppa E, Rossi FMV, Brandan E. Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro-adipogenic progenitors. J Cell Sci. 2019; 132(19):jcs232157.
- Juban G, Saclier M, Yacoub-Youssef H, Kernou A, Arnold L, Boisson C, Ben Larbi S, Magnan M, Cuvellier S, Theret M, Petrof BJ, Desguerre I, Gondin J, Mounier R, Chazaud B. AMPK Activation Regulates LTBP4-Dependent TGF-β1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy. Cell Rep. 2018; 25(8):2163-2176.e6
- Theret M, Gsaier L, Schaffer B, Juban G, Ben Larbi S, Weiss-Gayet M, Bultot L, Collodet C, Foretz M, Desplanches D, Sanz P, Zang Z, Yang L, Vial G, Viollet B, Sakamoto K, Brunet A, Chazaud B and Mounier R. AMPKα 1-LDH pathway regulates muscle stem cell self-renewal by controlling metabolic homeostasis.EMBOj 2017; 36(13):1946-1962.
- Chan KL, Pillon NJ, Sivaloganathan, DM, Costford SR, Theret M, Chazaud B, Klip A. Palmitoleate Reverses High Fat-induced Pro-inflammatory Macrophage Polarization via AMPK. J Biol. Chem. 2015; 290(27):16979-88.
- Gondin R, Theret M*, Duhamel G*, Pegan K, Mathieu JRR, Peyssonnaux C, Cuvellier S, Latroche C, Chazaud B, Bendahan D, Mounier R. Myeloid HIFs are dispensable for resolution of inflammation during skeletal muscle repair. J Immunol. 2015; 194(7):3389-99 *authors contributed equally
- Mounier R*, Theret M*, Arnold L, Cuvellier S, Bultot L, Göransson O, Sanz N, Ferry A, Sakamoto K, Foretz M, Viollet B, Chazaud B. AMPKα1 regulates macrophage skewing at the time of resolution of inflammation during skeletal muscle regeneration. Cell Metabolism. 2013; 18:251-64 *authors contributed equally
- Ali Al Ahmad MZ, Dubreil L, Chatagnon G, Khayli Z, Theret M, Martignat L, Chebloune Y and Francis F. Goat uterine epithelial cells are susceptible to infection with Caprine Arthritis Encephalitis Virus (CAEV) in vivo. Vet Res. 2012; 43:5
- Lesault PF*, Theret M*, Magnan M, Cuvellier S, Niy Y, Jacques T, Gherardi R, Hittinger L, Chazaud B. Macrophages improve survival, proliferation and migration of engrafted myogenic precursor cells into MDX skeletal muscle. PLoS One. 2012; 7:e46698 *authors contributed equally
- Rouger K, Larcher T, Dubreil L, Deschamps JY, Le Guiner C, Jouvion G, Delorme B, Leibeau B, Carlus M, Fornasari B, Theret M, Orlando P, Ledevin M, Zuber C, Leroux I, Deleau S, Guigand L, Testault I, Le Rumer E, Fiszman M, Chérel Y. Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchene muscular dystrophy dogs. Am J Pathol. 2011; 179:2501-18
Reviews
- Theret M, Saclier M, Messina G, Rossi FMV. Macrophages in skeletal muscle dystrophies, an entangled partner. J. Neuromuscular Diseases. Accepted 2021 Sep 17. doi: 10.3233/JND-210737
- Soliman H, Theret M, Scott W, Hill L, Underhill TM, Hinz B, Rossi FMV. Multipotent stromal cells: one name, multiple identities. Cell Stem Cell. 2021; 28(10):1690-1707.
- Contreras O, Rossi FMV, Theret M. Origins, potency, and heterogeneity of skeletal muscle fibro-adipogenic progenitors-time for new definitions. Skelet. Muscle. 2021; 11(1):16.
- Theret M, Rossi FMV, Contreras O. Evolving Roles of Muscle-Resident Fibro-Adipogenic Progenitors in Health, Regeneration, Neuromuscular Disorders, and Aging. Front Physiol. 2021; 12:673404.
- Theret M, Mounier R, Rossi F. The origins and non-canonical functions of macrophages in development and regeneration. 2019. Development. 2020; 146(9).
- Theret M, Gsaier L, Mounier R. AMPKα1-LDHA, a new metabolic pathway, regulating stem cell fate. Cell Cycle. 2018; 10:1-8.
- Mounier R, Theret M, Lantier L, Foretz, Viollet B. Expanding roles for AMPK in skeletal muscle plasticity. Trends Endocrinol Metab. 2015; 26(6):275-286
- Theret M, Chazaud B, Mounier R. [When regulation of cell energy meets regulation of inflammation: AMPK triggers skewing of macrophage phenotype during skeletal muscle regeneration]. Médecine/Sciences2014; 30: 35-8
Book Chapters
- Theret M, Gsaier L, Ben Larbi S, Weiss-Gayet M, Mounier R. Analysis of Muscle Stem Cell Fate Through Modulation of AMPK Activity. Methods Mol Biol. 2018;1732:539-549
- Saclier M*, Theret M*, Mounier R, Chazaud B. Effects of conditioned-medium on murine and human muscle cells. Methods Mol Biol. 2017;1556:317-327 *authors contributed equally
Preprint
- Caratti G, Desgeorges T, Juban G, Koenen M, Kozak B, Theret M, Chazaud B, Tuckermann JP, Mounier R. AMPKα1 is essential for Glucocorticoid Receptor triggered anti-inflammatory macrophage activation. doi: https://doi.org/10.1101/2020.01.02.892836
Position: Postdoctoral Research Fellow
Nationality: French
Research summary:
Beside its function as a biological barrier for infection, the immune system participates in multiple cellular process involved in tissue homeostasis during development, post-natal growth, regeneration/repair, and ageing. To do this, immune cells strongly interact with tissue-resident cells, such as Mesenchymal Progenitor (MPs). This is a two-way relationship, as MPs are also required to maintain organ function and tissue integrity, by strongly influencing immune cells inflammatory function. For example, a stable type 2 inflammatory (Th2) environment is an important, yet underestimated, parameter required for successful tissue maintenance. Indeed, white adipose tissue (WAT) resident MPs are responsible for the maintenance of a Th2 environment within the WAT at homeostasis. While the immune functions of MPs are actively studied, the signalling pathways involved in their regulation, which are likely to provide potential therapeutic targets, are so far poorly understood. MPs are the main cellular component of the tissue interstitial. Their role is to maintain the tissue homeostasis at resting state, but they are also crucial to keep the tissue integrity after a damage. While their function toward the ECM is well known, these cells have been more recently described as a key player of the inflammation.
During my PDF training, I have studied the implication of various signalling pathways involved on the inflammatory fate of the MPs.
Personal Summary:
Before moving to Vancouver, I would have described myself as a city person, enjoying museums, movies, and the night life. But the great outdoors of BC converted me to the west-coast life style (meaning that I cannot spend 2 days in a row without going to the gym, running, swimming, biking, hiking, camping, or driving across the province for a weekend). However (or luckily) my dry French humour is still very strong, and I still spend 60% of my time complaining.
Publications:
- Malakoutian M, Theret M, Yamamoto S, Dehghan-Hamani I, Lee M, Street J, Rossi F, Brown S, Oxland T. Larger Muscle Fibers and Fiber Bundles Manifest Smaller Elastic Modulus in Paraspinal Muscles of Rats and Humans. Sci Rep. 2021; 11(1):18565.
- Theret M*, Low M*, Rempel L, Li Fang Fang, Tung LW, Conreras O, Chang CK, Soliman H, Rossi FMV. In vitro assessment of anti-fibrotic drug activity does not predict in vivo efficacy in murine models of Duchenne muscular dystrophy. Life Sci. 2021; 279:119482. *authors contributed equally
- Yamamoto S, Malakoutian M, Theret M, Street J, Rossi FMV, Brown SHM, Oxland TR. The effect of posterior lumbar spinal surgery on passive stiffness of rat paraspinal muscles 13 weeks post-surgery. Spine (Phila Pa 1976). 2021; 46(21):E1125-E1135.
- White Z, Hakim CH, Theret M, Yang N, Rossi FMV, Cox D, Francis GA, Straub V, Selby K, Panagiotopoulos C, Duan D, Bernatchez P. High prevalence of plasma lipid abnormalities in human and canine Duchenne and Becker muscular dystrophies depicts a new type of primary genetic dyslipidemia. J Clin Lipidol. 2020; 14(4):459-469.e0.
- Contreras O, Soliman H, Theret M, Rossi FMV, Brandan E. TGF-β-driven downregulation of the transcription factor TCF7L2 affects Wnt/β-catenin signaling in PDGFRα+ fibroblasts. J Cell Sci. 2020; 133(12):jcs242297.
- McArthur S, Juban G, Gobbetti T, Desgeorges T, Theret M, Gondin J, Toller-Kawahisa JE, Reutelingsperger CP, Chazaud B, Perretti M, Mounier R. Annexin A1 drives macrophage skewing to accelerate muscle regeneration through AMPK activation J Clin Invest. 2020; 130(3):1156-1167.
- Contreras O, Cruz-Soca M, Theret M, Soliman H, Tung LW, Groppa E, Rossi FMV, Brandan E. Cross-talk between TGF-β and PDGFRα signaling pathways regulates the fate of stromal fibro-adipogenic progenitors. J Cell Sci. 2019; 132(19):jcs232157.
- Juban G, Saclier M, Yacoub-Youssef H, Kernou A, Arnold L, Boisson C, Ben Larbi S, Magnan M, Cuvellier S, Theret M, Petrof BJ, Desguerre I, Gondin J, Mounier R, Chazaud B. AMPK Activation Regulates LTBP4-Dependent TGF-β1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy. Cell Rep. 2018; 25(8):2163-2176.e6
- Theret M, Gsaier L, Schaffer B, Juban G, Ben Larbi S, Weiss-Gayet M, Bultot L, Collodet C, Foretz M, Desplanches D, Sanz P, Zang Z, Yang L, Vial G, Viollet B, Sakamoto K, Brunet A, Chazaud B and Mounier R. AMPKα 1-LDH pathway regulates muscle stem cell self-renewal by controlling metabolic homeostasis.EMBOj 2017; 36(13):1946-1962.
- Chan KL, Pillon NJ, Sivaloganathan, DM, Costford SR, Theret M, Chazaud B, Klip A. Palmitoleate Reverses High Fat-induced Pro-inflammatory Macrophage Polarization via AMPK. J Biol. Chem. 2015; 290(27):16979-88.
- Gondin R, Theret M*, Duhamel G*, Pegan K, Mathieu JRR, Peyssonnaux C, Cuvellier S, Latroche C, Chazaud B, Bendahan D, Mounier R. Myeloid HIFs are dispensable for resolution of inflammation during skeletal muscle repair. J Immunol. 2015; 194(7):3389-99 *authors contributed equally
- Mounier R*, Theret M*, Arnold L, Cuvellier S, Bultot L, Göransson O, Sanz N, Ferry A, Sakamoto K, Foretz M, Viollet B, Chazaud B. AMPKα1 regulates macrophage skewing at the time of resolution of inflammation during skeletal muscle regeneration. Cell Metabolism. 2013; 18:251-64 *authors contributed equally
- Ali Al Ahmad MZ, Dubreil L, Chatagnon G, Khayli Z, Theret M, Martignat L, Chebloune Y and Francis F. Goat uterine epithelial cells are susceptible to infection with Caprine Arthritis Encephalitis Virus (CAEV) in vivo. Vet Res. 2012; 43:5
- Lesault PF*, Theret M*, Magnan M, Cuvellier S, Niy Y, Jacques T, Gherardi R, Hittinger L, Chazaud B. Macrophages improve survival, proliferation and migration of engrafted myogenic precursor cells into MDX skeletal muscle. PLoS One. 2012; 7:e46698 *authors contributed equally
- Rouger K, Larcher T, Dubreil L, Deschamps JY, Le Guiner C, Jouvion G, Delorme B, Leibeau B, Carlus M, Fornasari B, Theret M, Orlando P, Ledevin M, Zuber C, Leroux I, Deleau S, Guigand L, Testault I, Le Rumer E, Fiszman M, Chérel Y. Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchene muscular dystrophy dogs. Am J Pathol. 2011; 179:2501-18
Reviews
- Theret M, Saclier M, Messina G, Rossi FMV. Macrophages in skeletal muscle dystrophies, an entangled partner. J. Neuromuscular Diseases. Accepted 2021 Sep 17. doi: 10.3233/JND-210737
- Soliman H, Theret M, Scott W, Hill L, Underhill TM, Hinz B, Rossi FMV. Multipotent stromal cells: one name, multiple identities. Cell Stem Cell. 2021; 28(10):1690-1707.
- Contreras O, Rossi FMV, Theret M. Origins, potency, and heterogeneity of skeletal muscle fibro-adipogenic progenitors-time for new definitions. Skelet. Muscle. 2021; 11(1):16.
- Theret M, Rossi FMV, Contreras O. Evolving Roles of Muscle-Resident Fibro-Adipogenic Progenitors in Health, Regeneration, Neuromuscular Disorders, and Aging. Front Physiol. 2021; 12:673404.
- Theret M, Mounier R, Rossi F. The origins and non-canonical functions of macrophages in development and regeneration. 2019. Development. 2020; 146(9).
- Theret M, Gsaier L, Mounier R. AMPKα1-LDHA, a new metabolic pathway, regulating stem cell fate. Cell Cycle. 2018; 10:1-8.
- Mounier R, Theret M, Lantier L, Foretz, Viollet B. Expanding roles for AMPK in skeletal muscle plasticity. Trends Endocrinol Metab. 2015; 26(6):275-286
- Theret M, Chazaud B, Mounier R. [When regulation of cell energy meets regulation of inflammation: AMPK triggers skewing of macrophage phenotype during skeletal muscle regeneration]. Médecine/Sciences2014; 30: 35-8
Book Chapters
- Theret M, Gsaier L, Ben Larbi S, Weiss-Gayet M, Mounier R. Analysis of Muscle Stem Cell Fate Through Modulation of AMPK Activity. Methods Mol Biol. 2018;1732:539-549
- Saclier M*, Theret M*, Mounier R, Chazaud B. Effects of conditioned-medium on murine and human muscle cells. Methods Mol Biol. 2017;1556:317-327 *authors contributed equally
Preprint
- Caratti G, Desgeorges T, Juban G, Koenen M, Kozak B, Theret M, Chazaud B, Tuckermann JP, Mounier R. AMPKα1 is essential for Glucocorticoid Receptor triggered anti-inflammatory macrophage activation. doi: https://doi.org/10.1101/2020.01.02.892836
